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. 2021 Jan 1;11(6):3000–3016. doi: 10.7150/thno.47354

Figure 4.

Figure 4

Deletion of ALKBH5 inhibits cardiomyocyte proliferation and cardiac regeneration after apex resection (AR) injury. (A) Schematic diagram for experimental procedure in ALKBH5 KO and wild-type (WT) mice. (B) HE staining of heart from P21 ALKBH5 KO mice and wild-type (WT) mice after treated with AR at P1. (C) Heart weight to body weight ratio of ALKBH5 KO mice and wild-type (WT) mice (n = 4). (D, E) Cardiac function analyzed by echocardiography. n = 6. **P < 0.01. (F) Wheat germ agglutinin (WGA) staining and quantification of P21 ALKBH5 KO (n = 13) and wild-type (WT) (n = 8) hearts after treated with AR at P1. **P < 0.01. Scale bars, 50 µm. (G) pH3 immunofluorescence staining in P21 ALKBH5 KO and wild-type (WT) hearts and quantification of pH3-positive CMs (9941 CMs in the AR-WT group and 6930 CMs in the AR-ALKBH5 KO group). ***P < 0.001. Scale bar, 50 µm. (H) Aurora B kinase immunofluorescence staining in P21 ALKBH5 KO and wild-type (WT) hearts and quantification of pH3-positive CMs (14038 CMs in the AR-WT group and 11287 CMs in the AR-ALKBH5 KO group). ***P < 0.001. Scale bar, 20 µm.