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Bulk quantitation of ddPCR products. (a) Total fluorescence of ddPCR emulsions measured with a plate reader (Tecan); (b) Detection of stained total DNA recovered from ddPCR emulsions (Qubit); (c) Quantitation of amplicon peak with gel electrophoresis (Bioanalyzer) of ddPCR emulsions. (d) qPCR quantitation of ddPCR amplicons. ddPCR+qPCR shifts the qPCR Ct to lower cycles, allowing enhanced sensitivity compared to qPCR alone. n = 3, error bars represent standard deviation.
