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. 2021 Jan 19;10(1):12. doi: 10.1038/s41389-020-00300-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Activated proteins in HT1080 monolayers and spheroids identified by human phospho-kinase array kit. B Western blot for indicated proteins following transduction with sh.Akt1/2 or sh.Scr in sarcoma spheroid cells. C CD133 (green) and Nanog (red) immunofluorescence with and DAPI (blue) counterstaining following treatment with PI3K inhibitor LY294002 or sh.Akt1/2 in spheroid cells. D Diameter of spheroids grown from single CD133+ cell cells showing following treatment with LY294002 or sh.Akt1/2 vs, DMSO or sh.Scr, respectively. E Proliferation of spheroid cells following treatment with sh.Akt1/2 and doxorubicin or 6 Gy radiation. β-Actin, loading control. Scale bar, 50 µm. *p < 0.01 compared to control.