Abstract
The rhesus macaque (Macaca mulatta) is the most widely studied nonhuman primate (NHP) in biomedical research. We present an updated reference genome assembly (Mmul_10, contig N50 = 46 Mbp), increasing the sequence contiguity 120-fold and annotate it using 6.5 million full-length transcripts, thus improving our understanding of gene content, isoform diversity, and repeat organization. With the improved assembly of segmental duplications, we discover novel lineage-specific genes and expand gene families that are potentially informative in studies of evolution and disease susceptibility. Whole-genome sequence data from 853 captive rhesus macaques identifies polymorphism in 85.7 million single-nucleotide and 10.5 million indel variants, including potentially damaging variants in genes associated with human autism and developmental delay, providing a framework for developing non-invasive NHP models of human disease.
Summary:
A compendium of rhesus macaque genome variation
A detailed understanding of nonhuman primate (NHP) genome evolution is key to recognizing the origins of human traits and identifying putative disease genes. Evolutionary analyses of a diverse range of NHP genomes, spanning the breadth of primate phylogeny from great apes to prosimians, have begun to uncover the genetic basis of this phenotypic and biochemical diversity. Comparisons among species reveal lineage-specific changes in retroelements, the death and birth of duplicated genes, including the segmental duplications (SDs) underlying them, and functionally significant, sometimes deleterious, mutations in genes associated with human disease (1) (2) (3) (4) (5). Collectively, these studies are beginning to illuminate the history and novel mechanisms of molecular and phenotypic adaptation.
Rhesus macaques play a unique and critical role in both evolutionary comparisons and biomedical research. While the great apes (chimpanzees, bonobos, gorillas and orangutans) are phylogenetically closer to humans, the rhesus macaque is an essential model for a wide variety of studies related to infectious disease, neurobiology, developmental psychology, and other elements of primate (including human) biological function (6). This significance is highlighted by the role that rhesus macaque models have played in our understanding of AIDS pathogenesis and prevention strategies such as pre-exposure prophylaxis regimes (7), the development of highly effective Ebola vaccines (8), and the striking results obtained by editing genes related to risk for autism (9).
In 2007, the first whole-genome analysis of the rhesus macaque genome revealed both fundamental genetic similarities as well as interesting differences from the human genome (2). By combining our new rhesus reference assembly with the high-quality genomes now available for the great apes (5), more extensive reconstruction of human genome evolution is possible, such as gene structural changes that are unique to humans and apes. Early analyses suggested that macaques showed reduced SD content and complexity when compared to human although a final determination required a higher quality genome assembly (2). Similarly, initial analyses reported an expanded and more complex major histocompatibility complex (MHC) loci in macaques but the organization of such loci has been difficult to resolve (10). Finally, macaques provide valuable models for diseases or processes that would not be adequately modeled in rodents(11–13). Naturally occurring variation, which is higher among macaques (3) (4), has been leveraged to develop improved genetic models of Mendelian disorders (14) (15), complex disease (16) (17), and a hereditary form of cancer(11). To improve our understanding of rhesus macaque genetic diversity and its future translational implications, we annotate this new macaque reference by extensively characterizing genomic variation among 853 Indian- and Chinese-origin rhesus macaques from US research colonies. Consequently, this work provides a roadmap for naturally occurring mutations and disease models.
Results
Sequencing and assembly.
We generated long-read sequence data (~66-fold single-molecule, real-time [SMRT] sequence coverage) and assembled the genome of a female Indian-origin rhesus macaque (2.9 Gbp) using a series of genomic methods followed by extensive manual curation (18). This reference (Mmul_10, GenBank accession GCA_003339765.3) consists of 20 autosomes and the X chromosome; for completeness, we added a previous bacterial artificial chromosome (BAC)-based representation of the Y chromosome (19). Only 3.3% (97 Mbp) of the assembled macaque genome remains unassigned to scaffolds and is highly repetitive (>85% repeat-masked bases >10 kbp in length). In total, the new macaque reference is represented by 2,979 scaffolds with contig and scaffold N50 lengths of 46 and 82 Mbp, respectively (Table 1). This resulted in a highly contiguous and accurate assembly (Fig. 1) owing to extensive manual curation, gap filling, and unique sequence directionality assessments (18). Overall contiguity increased by 120- and 5.6-fold when compared to the previous Indian (Mmul_8.0.1) and Chinese rhesus macaque (rheMacS) (20) assemblies, respectively (Table 1). The underlying raw sequence data and other data are available at NCBI (Table S1).
Table 1.
Genus species | Assembly version | N50 contig (Mbp) | Total size (Mbp) | Total contigs | Chromosome gaps1 | Unplaced bases (Mbp) | Protein-coding genes | % Missing genes3 |
---|---|---|---|---|---|---|---|---|
Macaca mulatta | Mmul_8.0.1 | 0.107 | 2,835 | 348,579 | 62,231 | 82 | 21,574 | 3.8 |
Macaca mulatta | rheMacS_1.02 | 8 | 3,031 | 4,713 | 2,862 | 82 | 20,389 | 1.8 |
Macaca mulatta | Mmul_10 | 46 | 2,936 | 3,182 | 203 | 97 | 21,120 | 0.4 |
Quality assessment.
We performed a series of analyses to evaluate the quality of the Mmul_10 reference assembly. For example, we assessed accuracy and contiguity using macaque BAC-end sequence (BES) data estimating values of 99.7% and 92.5%, respectively, consistent with allelic variation among individual macaques. Detailed analyses of even complex regions such as the MHC genes (10) and the killer immunoglobulin-like receptor (KIR) gene families (21) show that the majority of these loci were assembled accurately (Tables S2 and S3; (18)). Using orthogonal sequencing datasets from the same sample, we estimate an overall assembly sequence accuracy of one error every 20,000 base pairs.
Over 99.7% of the gaps present in the previous Indian-origin rhesus macaque genome assembly are now closed. Eleven of the 20 macaque autosomes are now represented as two scaffolds separated only by an unassembled centromere. While there is high overall synteny with Mmul_8.0.1, rheMacS, and macFas_5.0 (chromosome 3 [Figs. 1B; S1]), there are 39 large orientation errors identified in the original Mmul_8.0.1 assembly that our assembly is able to correct (22) (chromosomes 13 [Fig. S2] and 2 [Fig. S3]). In order to develop a more accurate assembly for this species, we investigated other potential orientation issues by generating Strand-seq data from an unrelated macaque (18), compared it to the two Indian macaque assemblies, and identified “homozygous inversions” as potential errors in orientation. In total, we detected 82 (130,115,998 bp) potentially misoriented regions in Mmul_8.0.1 in contrast to 13 (3,800,615 bp) in the new reference assembly (Fig. 1C; Tables S4 and S5). Importantly, these data indicate that the number of misoriented genes has been reduced from 4.83% to only 0.13% in Mmul_10. Many of these unresolved regions, not surprisingly, map to structurally diverse and complex immune gene families, such as the MHC and KIR regions, where in the latter a short homozygous inversion is predicted (Fig. S4). The Strand-seq analysis also detected one chimeric scaffold where a terminal part of chromosomal scaffold CM014356.1 belongs to the beginning of chromosomal scaffold CM014355.1 (Fig. S5), which has now been corrected.
Gene annotation.
We assessed the completeness of gene annotation by applying benchmarking universal single-copy ortholog (BUSCO) scores, which measure the representation of highly conserved mammalian genes (23). We find that 99.6% of BUSCO genes are annotated with only 0.1% missing—an improvement over the Chinese macaque assembly (rheMacS) where 1.8% are missing (20) (Table S6). Analyzing a curated set of 6,422 Macaca mulatta RefSeq transcripts (NCBI) shows an average coverage of 99.8% confirming the high degree of completeness. Predicted protein-coding gene comparisons within each annotation pipeline, NCBI and Ensembl, for rhesus macaque and human show consistency (Table S7). In rhesus macaque, NCBI and Ensembl produced similar outcomes: 21,121 and 21,748 genes, respectively (Table S7). We note a substantial improvement in ncRNA identification in Mmul_10, with 8,720 new ncRNAs (Table S7), especially lncRNA (Tables S8 and S9; (18)). Many of the missing genes map to more complex regions of the genome or duplicated genes that have expanded or contracted differentially when compared to human (Tables S10 and S11).
In an effort to further define macaque-specific transcript and protein isoform diversity, we generated and sequenced 6.5 million full-length cDNAs from macaque brain, induced pluripotent stem cell (iPSC) lines, and testes (Table S12). We applied the Comparative Annotation Toolkit (CAT) (24) to annotate 17,838 protein-coding and 31,873 noncoding macaque genes. The set includes 83,692 protein-coding isoforms, of which 5,353 were identified from Iso-Seq data as potentially novel isoforms (Fig. S6). A total of 980 genes have frameshifting indels disrupting all isoforms; RNA-seq-based cleanup reduced the proportion of isoforms with frameshifting indels from 16.5% to 4.9%. The final CAT gene set includes 80,248 protein-coding transcripts aligned in a 1–1 fashion, with the remaining 3,444 paralogous isoforms being resolved with alignment metrics. We note that 550 gene structures are split over multiple contigs (Table S13) and 827 protein-coding genes show evidence of being part of gene families exhibit reduced copy number in this assembly relative to human, with 473 of those showing a 2 to 1 relationship and 295 being 3 to 1 in human when compared to macaque (Table S10). In contrast, 967 protein-coding genes show evidence of gene family expansion in macaque, with 711 copied once and 116 copied twice in rhesus when compared to humans (Table S11).
Novel exon adaptation in macaque.
CAT predicted 2,880 novel transcripts that did not arise from any previously annotated transcript in the input human annotation. Of these, 2,812 maintain open reading frames. From this set, we manually curate 84 novel exons with Iso-Seq support (Fig. 2; Table S14). We searched the translated protein sequence in the Pfam 32.0 database (25) using this set and found significant homology to notable protein families. For example, three transcripts (Rhesus_T0212625, Rhesus_T0212626, and Rhesus_T0212627) correspond to a novel gene model (Rhesus_G0055137 on chromosome 9:95,447,150–95,611,700) that shares homology with human CYP2C18 protein and has abundant Iso-Seq transcript support across multiple macaque tissues (Fig. 2A). The predicted mRNA sequence for tropoelastin (ELN) has two identifiable exons near the C-terminus of the protein with Iso-Seq support from six tissues for three alternatively spliced isoforms of ELN. These exons are shared to the base of the mammalian tree, but not found in humans, great or lesser apes, suggesting a unique loss of these exons in the hominoid lineage (Fig. 2B).
We explored additional exon adaptations that were potentially unique to rhesus macaque. The first is a deletion of 64 bases in the macaque genome affecting MYO3A (Fig. S7A). This deletion leads to a new isoform of MYO3A with altered exon structure and is supported by Iso-Seq data from multiple tissues. Second, is an isoform of GAS8 where a novel exon leads to a frameshift in downstream exons creating a premature stop codon that is alternatively spliced as confirmed by Iso-Seq (Fig. S7B). The third example is a rhesus-specific 6,250 base-pair insertion in DCHS2 that introduces a novel exon to one of the DCHS2 isoforms (Fig. S7C). Once again, this novel DCHS2 exon is supported by Iso-Seq reads from testes tissue where it is alternatively spliced. However, the Iso-Seq transcripts do not support the original CAT gene annotation predictions as a whole. Rather than a predicted exon skipping event, the novel exon appears to correspond to an alternate first exon of the gene. Additional experimental work will be required to determine the functional impact of these genic differences.
Segmental duplication analyses.
We analyzed Mmul_10 for recent duplications (18) and identified 111.5 Mbp of assembled SDs (≥1 kbp and ≥90% sequence identity). In principle this represents a >3-fold improvement compared to the analysis of the first macaque assembly (2) where only 32 Mbp were characterized as SDs. Despite this improvement, 54% of the duplicated base pairs remain unlocalized (Figs. S8 and S9). Nevertheless, the vast majority of those assigned to a chromosome are clustered or distributed interchromosomally among pericentromeric and subtelomeric regions (Fig. S10). Only 8% (755/9,475) of the pairwise alignments are intrachromosomal and separated by at least 1 Mbp (Fig. S10), for example, the collapsed SD of NXF2 on the X chromosome (Fig. S11).
We identified 276 regions of collapsed duplications (26) corresponding to 9.1 Mbp of the genome (Table S15) and estimate that these correspond to 41.8 Mbp of SDs not yet properly integrated into the macaque assembly. FISH analyses classified the majority of these (74%) as pericentromeric based on signals mapping to either side of the centromere. In order to resolve the sequence of these collapses, we applied a graph-based approach (26) (27) to resolve SDs based on clustering and assembling reads using diagnostic paralogous sequence variants. This method resolved 168 of the 276 collapses into 531 distinct contigs representing 19.8 Mbp of SD sequence (contig N50 = 37.4 kbp). Among these are highly accurate sequence contigs corresponding to recently expanded rhesus macaque gene families, including MHC, olfactory receptor, and zinc finger genes. We have deposited these contigs into GenBank under BioProject PRJNA662298 as a resource where they may be used to improve gene annotation. For example, we identified nine assembly collapses (20–92 kbp) corresponding to ZNF669 genes in the Mmul_10 assembly (Table S15). Segmental Duplication Assembler (SDA) assembles 53 contigs from these collapses generating an additional 1.9 Mbp of assembled sequence (N50 = 36.5 kbp) and identified three contigs where ZNF669 Iso-Seq mapped with higher identity than the original Mmul_10 assembly (Fig. 3A–B). Translation of the full-length cDNA confirmed open reading frames and duplicated gene models that had been missed by our initial annotation of the genome (Fig. 3C). FISH analysis of a large-insert BAC corresponding to one of the loci confirmed a cluster of ZNF669 genes mapping to chromosome 6 as well as several additional duplicated loci of this gene family distributed throughout the macaque genome (Fig. 3D).
Repetitive sequence analyses.
Overall, fewer mobile element insertions (MEIs) were identified in Mmul_10 when compared to Mmul_8.0.1((18); Table S16), including lineage-specific elements across various retrotransposon classes (Table S16). Despite these reductions, subfamily network analyses for both Alu (Fig. S12) and LINE 1 elements show increases in the number and connectivity of younger subfamilies, particularly full-length L1 (Fig. 4A–B). Notably, there is an increase in full-length potentially active L1 elements (n = 6,892 vs. n = 4,380) in Mmul_10 compared to Mmul_8.0.1 (Table S16). Full-length L1 elements are less fragmented and more likely to be assigned to chromosomes as opposed to being mapped to unlocalized contigs (Fig. 4C). Similarly, the new assembly moves 8,291 unlocalized Alu elements to specific chromosomal assignments (Fig. 4D). Interestingly, 33% of potentially full-length endogenous repeat elements (LTR > 7 kbp in length) now map to different chromosomal locations in the newer assembly (Fig. S13) consistent with improvements in the sequence resolution and integration of longer and potentially active repeat elements.
Recurrent deletions in full-length L1RS elements.
Given the better representation of full-length repeats, we searched for systematic changes in the 5’ UTR (untranslated region) structure of the primate-specific L1PA subfamily from which L1 rhesus-specific (L1RS) retroelements originate (28). The 5’ UTR of these selfish elements are often targeted by host factors, e.g., KRAB zinc finger (KZNF) proteins, which repress their transcription and, as a result, are differentially expanded across primate lineages (29). We mapped 20,541 full-length L1RS elements to the human L1PA5 consensus sequence (UCSC Repeat Browser) and identified specific coverage drops that accrue and persist in subsets of L1RS elements (28). Using these deletion patterns and subfamily designations, we propose an order for the evolution of these 5’ UTR deletion events (Fig. 5A) and suggest a reclassification of L1RS nomenclature. Although older families are typically assigned a higher number, the appearance of coverage drops establishes that the L1RS16 family predates the L1RS21 family (Fig. 5B). Furthermore, the results indicate that after the human–rhesus divergence, at least three different regions of the L1RS 5’ UTR were altered. A comparative analysis of these L1RS elements in the genomes of other Old World monkeys (OWMs; rhesus, crab-eating macaque, baboon, and golden snub-nosed monkey with human as an outgroup) supports this adaptive model (Fig. 5B), as all OWM species display changes at these positions, although the actual sequence changes and size of the region varies, suggesting that these events recur or have been refined at different points along the OWM phylogenetic tree (Fig. S14). Two of these three sites (Site 1 and Site 3) also overlap changes observed in active human-specific L1 elements supporting the hypothesis that the deletions result from independent recurrent parallel evolution in the primates. The remaining site (Site 2) is restricted to OWM as no coverage drop is observed at this site in young full-length L1 human elements. The existence of a deletion at this site in all OWMs suggests that a repressive factor was present in the OWM common ancestor and highlights the uniqueness of L1RS2 transcript diversity (Fig. 5C).
Macaque genetic diversity among research populations.
The US research colonies of rhesus macaques were founded primarily with animals imported from India decades ago, although a much smaller number of Chinese-origin rhesus macaque have been added to some colonies over time. It is not possible in all cases to trace the exact geographic origins of the US research population, but sufficient information is available to identify many animals as derived from either Indian- or Chinese-origin founders. We generated whole-genome sequence (WGS) data for 850 rhesus macaques from captive US research colonies and three wild-caught Chinese samples, including 133 previously published samples (3). The majority of the samples (n = 810) were designated as Indian origin whereas the remaining individuals were of Chinese or suspected admixed origin. Most samples were sequenced to at least 20-fold coverage (n = 764; average 33.69-fold) with the remaining (n = 89) sequenced to an average of 8.58-fold for the detection of single-nucleotide variants (SNVs) and indels (Figs. S15 and S16). SNVs and indels were identified based on mapping reads to Mmul_10 (18) with an overall 2.12 Ts/Tv ratio consistent with prior studies (3). We identified 85.7 million SNVs, including 21.3 million singletons in addition to 10.5 million indels (Table 2), creating the most extensive collection of segregating genetic variants for any NHP species (Table S17). By comparison, a recent study of 929 human genomes from 54 diverse global populations, sequenced to average 35-fold coverage, identified just 67.3 million SNVs (30). Thus, the research rhesus macaques were more than twice as diverse per individual as humans with the average macaque carrying 9.7 million SNVs.
Table 2.
Type | Total variants | Singletons | Multiallelic variants |
---|---|---|---|
All SNVs | 85,721,160 | 21,270,272 | 3,160,393 |
All indels | 10,501,197 | 2,956,760 | 1,875,826 |
Protein-coding SNVs | 790,377 | 222,766 | 23,335 |
Protein-coding indels | 33,823 | 13,227 | 3,663 |
LGD SNVs2 | 20,400 | 6,570 | 1,026 |
LGD indels3 | 26,774 | 10,330 | 3,051 |
SNV and indel protein-coding classifications based on Ensembl Variant Effect Predictor (VEP)
Likely gene disruptive (LGD) SNVs
LGD indels defined as VEP consequences splice_acceptor_variant, splice_donor_variant, stop_gained, stop_lost, start_lost
A principal component analysis (PCA) of the SNV genotypes readily discriminated between the Chinese and Indian rhesus macaques (Fig. 6A). Thirty-one individuals that were initially identified as Indian origin show some degree of Chinese rhesus admixture, although the extent of admixture varies considerably (Fig. 6A). The free-ranging Cayo Santiago rhesus macaque population (Caribbean Primate Research Center) shows a gradient of variation with respect to other Indian rhesus macaques (PC2)—likely a consequence of a genetic bottleneck since its initial founding in 1938 on the Puerto Rican Island of Cayo Santiago (31). Consistent with this observation, the Cayo macaque population shows lower heterozygosity and larger runs of homozygosity when compared to other National Primate Research Center (NPRC) populations (Figs. S17–19). Interestingly, a preliminary analysis shows that linkage disequilibrium (LD) decays more rapidly among unrelated Indian rhesus macaques than in a subset of the human African population, but at greater physical distance (>50 kbp) the macaques retain higher linkage disequilibrium (Fig. S20).
We repeated the PCA excluding both the Cayo and Chinese macaque populations (Fig. 6B). In this analysis macaque genetic variation from most primate research centers was indistinguishable with the exception of Oregon National Primate Center (ONPRC), Yerkes Primate Research Center (YPRC), and California National Primate Research Centers (CNPRC) for which subsets of individuals appear genetically distinct (Fig. 6B). A population structure analysis shows that the CNPRC macaques have a somewhat greater admixture with Chinese macaques than other populations on the basis of the genomes analyzed here (Figs. S21 and S22). In addition, within some of the research populations (e.g., YNPRC, Cayo, etc.), genetically distinct subgroups of animals are identified with some consistent substructure.
Analysis of variants for functional changes.
Macaques are important models of human genetic disease (3) (4) so we investigated both common and rare variants from our samples that affect the sequences of protein-coding genes. In total, we identified 85.7M (million) SNVs of which 3.2M were multiallelic and 21.3M were singletons, as well as 10.5 indels (Table 2). For protein-coding sequences we find 790,377 SNVs and 33,823 indels (Table 2). Based on Variant Effect Predictor annotations, we identify 408,496 missense and 20,400 likely gene-disruptive (LGD) mutations (Table S18, Table 2). Of all variant classes, the LGD mutations occur at the lowest frequency (0.001—0.01) consistent with a more deleterious effect on phenotype (Fig. 6C). An assessment of homozygous-damaging mutations shows considerable overlap among the NPRC colonies for more common variants while a smaller subset is unique to each (Fig. S23). We generated a summary distribution of all missense variant counts per gene and normalized by the gene length as defined by the number of protein-coding bases in the gene, excluding introns and UTRs (Fig. S24).
To illustrate the biological potential of the macaque genetic diversity, we identified naturally occurring macaque mutations in orthologs of human genes implicated in autism and developmental delay (32) (33). In humans, de novo deleterious mutations in these genes are thought to be dominant and have a large effect, but mouse models often do not recapitulate the complexity of neurobehavioral features of human disease (32). We considered all missense and LGD mutations such as frameshift, stop, and splice site mutations that would disrupt protein-coding sequence. We further classified the genes on the basis of their intolerance to gene-disruptive mutation (pLI or the probability of being loss-of-function intolerant) (Figs. 6D, S25 and S26). The pLI score has been widely adopted and is based on the number of observed versus expected protein-truncating variants in a population. The closer the pLI score is to 1, the more intolerant to variation the gene is predicted to be. Revisiting gene annotation (based on the Iso-Seq resource) shows that while some of these correspond to changes in gene annotation between species, other changes represent viable candidates to establish new models of neurodevelopmental disease (e.g., severe missense mutations in ARID1B). Neurodevelopmental delay genes are significantly depleted for missense variants when compared to all genes (p = 8.883e-41; Fig. S25A). Remarkably, we identified nine genes with candidate deleterious mutations in macaque that are intolerant to mutation in humans and where de novo mutations in human are associated with neurodevelopmental disorders (Table S19). Homozygous LGD variants segregating in rhesus macaque research centers offer even more opportunities for exploring their biological relevance among NHPs (Table S20).
Finally, structural variation differences also present an opportunity to develop new rhesus macaque disease models as well as enhance our understanding of fixed and polymorphic changes between species. For example, we identified ~301,000 insertions and 241,000 deletions shared between the Indian- and Chinese-origin macaques (Fig. S27; Tables S21 and S22). Of these 542,000 insertion or deletion events, only a small fraction (1.68%) are predicted to affect genes (Table S22). Among the 87,227 structural variants unique to either subspecies, we predict that 1,614 may affect genes although validation in addition to genotyping and genome assembly of more individuals (especially wild caught) will be required to establish fixed differences between the Chinese and Indian macaque genomes.
Discussion
The rhesus macaque is arguably the most important NHP for biomedical research and is a key species in the study of primate evolution. The resources we developed and present here will significantly advance both areas by providing new biological insights and an improved framework for future gene-based disease research. In this new assembly we have corrected indel errors and misassemblies, properly represented inverted sequences, and flagged several remaining orientation issues. The various orthogonal technologies employed in the data production process, coupled with detailed manual curation, led to dramatically improved gene annotation, eliminated 99.7% of gaps and reduced misorientations when compared to the previous Indian-origin macaque assembly (2).
This new reference genome identifies previously unknown genes and genes whose intron/exon structure differs in comparison to humans, such as the 3.1 kbp Alu-mediated deletion that removed two protein-encoding exons from tropoelastin (ELN) relative to the human and ape orthologs (Fig. 2). Similar to finishing efforts for the mouse and human assemblies (34) (35), most of the novel macaque and OWM genes occur among duplicated gene families (Fig. 3). Indeed, our estimate of recent SDs has nearly doubled for this assembly (130–140 Mbp) and is beginning to approximate that of human (5–6%) (36) (37). Unlike human, however, where a large fraction of the SDs are interspersed along chromosomal arms, most macaque duplications appear either clustered or pericentromeric, bracketing the centromeres of macaque chromosomes. Similar to SDs, repeat content, especially for full-length elements, has facilitated comparative analyses identifying recurrent deletion events in the 5’ UTR of L1 elements in human and multiple OWM lineages as a potential adaptive response helping to evade KRAB-ZNF repression. It is tempting to speculate that expansion of OWM (including rhesus macaque) ZNF genes such as ZNF669 are part of an ongoing arms race to suppress new rhesus L1 subfamilies (38). While the assembly we generated is superior by most metrics, we recognize that centromeres, acrocentric regions, and some of the largest SDs still remain unresolved or unassigned.
Using this new macaque reference, we establish an extensive SNV resource that will facilitate future genetic analyses of biomedical research colonies. Our catalog of thousands of naturally occurring common missense variants and identification of other rare macaque mutations may help the discovery of new models of disease, such as those implicated in autism and human neurodevelopmental disorders (32) (33). These naturally occurring mutations provide an opportunity to develop noninvasive models of human disease without the expense of CRISPR-engineering of embryos (9). These models may be particularly powerful in relation to phenotypes that are not readily reproduced in non-primate knockout models (12) and for evaluating the effect of genetic variation on the efficacy of treatments prior to human trials.
Methods summary
A single female rhesus macaque of Indian origin (AG07107) was sequenced, assembled, and manually curated with a physical map, proximity ligation, and Strand-seq sequence data. Full-length cDNA was prepared from various tissue sources and used to annotate the genome assembly with three independent gene annotation pipelines: NCBI, Ensembl, and CAT. A whole-genome analysis comparison coupled with read-depth assessment was used to estimate the proportion of SDs. We used interphase and metaphase FISH on a female rhesus macaque lymphoblast cell line to validate SD order and orientations. All repeat elements were quantified using RepeatMasker for comparisons among rhesus macaque assemblies. To estimate rhesus macaque genetic diversity, we sequenced 853 animals across nine US rhesus macaque research colonies using standard Illumina sequencing instruments. We used a compendium of best practices to call sequence variants for SNVs, insertions, deletions, and structural variants. To classify the potential impact of SNVs, we aligned all to the human genome and focused our analysis on those that cause loss of function by stop-gain, start-lost, splice-donor or -acceptor base changes and missense variants that alter amino acid coding of the protein. A subset of these SNVs were compared to human genes known to harbor rare de novo deleterious variants that have been implicated in human neurodevelopmental disorders.
Supplementary Material
Acknowledgements:
We thank staff at the NPRCs involved in the preparation of biomaterials used for WGS; Sam Peterson (OHSU) for preparation of mRNA used in the generation of Iso-Seq datasets; and specifically, the UC Davis Primate Research Center for the rhesus macaque fetal brain material used for Iso-Seq data production. We would like to thank the Baylor College of Medicine Human Genome Sequencing Center production teams, especially Donna Muzny and Harshavardhan Doddapaneni, for their work on macaque diversity sequencing, and the Baylor Human Genome Sequencing Center, the Texas Advanced Computing Center, and Rice University for allowing us to use their computational resources for mapping and variant calling. The cell line MMU1 used for Strand-seq and FISH analyses was obtained from the Department of Comparative Genetics and Refinement, Biomedical Primate Research Centre (BPRC), Netherlands (courtesy of Ronald E. Bontrop). Alex Pollen (UCSF) and Sam Peterson (OHSU) for preparation of mRNA used in the generation of Iso-Seq datasets; and specifically, the UC Davis Primate Research Center for the rhesus macaque fetal brain material and specific tissues from developmentally staged samples used for Iso-Seq data production.
Funding: This work was supported, in part, by the National Institutes of Health (NIH) grants: HG002385 and 1U24HG009081 to E.E.E.; U01HG010961, U41HG010972, R01HG010485, 2U41HG007234, U01HL137183, 5U54HG007990 and 5T32HG008345-04 to B.P.; R01MH081884, R01MH046729 and P50MH100031 to N.H.K.; R01GM59290 to M.A.B.; by a subagreement from European Molecular Biology Laboratory with funds provided by agreement number 2U41HG007234-05 from National Institute of Health, NHGRI. Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NIH, NHGRI, or European Molecular Biology Laboratory. Rhesus WGS from primate research centers was supported by NIH grant 5R24OD011173 to J.R.; R24OD021324 grant that supported sequencing of the ONPRC cohort to B.F.; P51-OD011106 grant that supported sequencing of the WNPRC cohort as well as support for D.O., J.R., N.K., D.A., and J.K.; CNPRC base grant (P51OD011107) and BBA grant OD010962 to J.P.C.; YNPRC Pilot Research Project Program to Z.A.K-B. and its base grant P51OD011132; TNPRC grants P51OD011104, U42OD024282 and U42OD010568; 1R01HG010329 to S.R.S.; R01HG002939 to M.A.B. provided support for repeat analyses. E.E.E. is an investigator of the Howard Hughes Medical Institute.
Footnotes
Competing interests: The authors declare no competing financial interests.
Data availability: All data is available in the main text or the supplementary materials. The Mmul_10 genome assembly is available in the NCBI assembly archive under accession number GCF_003339765.1.
References and notes
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