Fig. 1.

Single‐cell analysis of sinusoidal cells from healthy and fibrotic mouse livers. (A) Whole‐liver RNA‐sequencing of mice treated with CCl₄ or veh (n = 2‐3). Z‐scores of 295 differentially expressed genes (4w CCl₄ vs. veh, P_adj < 0.05, DESeq2). Seven hierarchical clusters are indicated with enriched GO‐categories (P_adj < 0.05) and exemplary genes of each category (N/A: No significantly enriched category for cluster). (B) Sirius red, αSMA, CD34 and F4/80 IHC of representative livers from mice treated as indicated. (C) Centre; UMAP of >35K single cells colored according to cell type. Right; UMAPs showing log₂‐expression of marker genes. Bottom; UMAPs indicating treatment group of each cell. (D) Scaled log₂‐expression of marker genes in all cells grouped by cell type. (E) Scaled, log₂‐expression of select cell type‐specific and injury‐responsive genes stratified by cell type and treatment group (v = vehicle). (F) IF of CD34 (red) and αSMA (green) in livers of 4w CCl₄‐treated mice. Arrows indicate double‐positive cells shown at 4x magnification.