Fig. 3.

Sinusoidal dynamics and interactions in healthy and fibrotic livers. (A) UMAPs of 17748 LECs with indication of treatment groups and louvain clustering (PN: portal node‐proximal LECs; CV: central vein‐proximal LECs). Lower panel shows the representation of each treatment group in the louvain clusters 1‐6. Counts normalized by total LEC counts in each treatment group. (B) Log₂‐expression of cluster‐selective marker genes and select injury‐responsive genes across LEC louvain clusters 1‐5. Right; UMAPs of LECs showing log₂‐expression levels of select genes. (C) UMAPs of 6611 KC/MDMs with indication of treatment groups and louvain clustering. Lower panel showing the representation of each treatment group in the louvain clusters 1‐6. Counts normalized by total KC/MDM counts in each treatment group. (D) Log₂‐expression of cluster‐selective marker genes and select injury‐responsive genes across KC/MDM louvain clusters 1‐4. Right; UMAPs of KC/MDMs showing log₂‐expression of select genes. (E) Row‐normalized z‐scores of select marker genes and proliferation‐associated genes in KC/MDM louvain clusters 1‐3 and 5. (F) Directional cell‐cell interactions inferred from ligand‐receptor pairs and stratified by louvain clusters. Left; HSC‐to‐LEC/KC/MDM interactions. Right; LEC/KC/MDM‐to‐HSC interactions. Circle size indicates enrichment of expression of interacting partners in the interacting cluster pairs and color indicates mean expression of interacting partner subunits. Applied mean expression cut‐off is ≥3 for at least one interacting cluster pair.