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. 2020 Dec 25;13(1):25. doi: 10.3390/v13010025

Figure 5.

Figure 5

Subcellular localization (A,B) and bimolecular fluorescence (BiFC) (C) assays of P4 and P5 of jujube yellow mottle-associated virus (JYMaV) in epidermal cells of wild-type Nicotiana benthamiana leaves. The fusion proteins CMV3a-mCherry and H2B-mCherry were used as plasmodesmata and nucleus markers, respectively. Colocalization dots of JYMaV-P4 with CMV3a-mCherry at PD were indicated by arrow heads. Images were acquired 3 days after agroinfiltration under a confocal microscope at 63x/1.20 WATER objective. Scale bar = 20 μm.