Figure 1.

Schematic illustration of the isolation and purification of anthocyanins from black chokeberry fruits. The anthocyanins were isolated by an ultrasonic extraction method and the crude extract was prepared by XAD-7HP macroporous resins as the feed of simulated moving bed chromatography (SMB). The separation zones of SMB were zones II and III, and the solid phase was regenerated in zones I and IV with two columns per zone. The liquid phase (black) consisted of feed and desorbent for inlets, and extract and raffinate for outlets by pump E and pump R. The liquid phase was continuously fed the crude extract by pump F and eluted with ethanol by pump D. Then, SMB ACN was collected from the extract. The columns switched counter-clockwise (gray) at the switching time intervals.