Table 2.
Primary antibodies.
| A | |||
|---|---|---|---|
| Antibody | Host | Supplier, Cat#, RRID 1 | Concentration |
| Aldh1L1 | rabbit | Sigma-Aldrich, HPA036900, RRID:AB_10672273 | 1:200 |
| CNPase | rabbit | Synaptic Systems, 355 002, RRID:AB_2620111 | 1:500 |
| GFAP | chicken | Millipore, AB5541, RRID:AB_177521 | 1:500 |
| GFAP Al488 | mouse | Cell Signaling Technology, 3655, RRID:AB_2263284 | 1:100 |
| GS | goat | Santa Cruz Biotechnology, sc-6640, RRID:AB_641095 | 1:500 |
| Iba1 | goat | Abcam, ab5076, RRID:AB_2224402 | 1:1000 |
| NeuN | guinea pig | Synaptic Systems, 266004, RRID:AB_2619988 | 1:2000 |
| NG2 | rabbit | Millipore, AB5320, RRID:AB_91789 | 1:500 |
| Rab6A 2 | mouse | Sigma-Aldrich, WH0005870M1, RRID:AB_1843236 | 1:5000 |
| Sox9 | goat | R and D Systems, AF3075, RRID:AB_2194160 | 1:500 |
| B | |||
| Antibody/Reagent | Supplier | Concentration | |
| Donkey-anti-chicken-AMCA | Jackson Immunoresearch | 1:100 | |
| Donkey-anti-rabbit-Alexa 488 | Jackson Immunoresearch | 1:100 | |
| Donkey-anti-mouse-Alexa 488 | Jackson Immunoresearch | 1:100 | |
| Donkey-anti-mouse-Alexa 647 | Jackson Immunoresearch | 1:100 | |
| Donkey-anti-mouse-Cy3 | Jackson Immunoresearch | 1:1000 | |
| Donkey-anti-goat-Dy 488 | Jackson Immunoresearch | 1:100 | |
| Donkey-anti-guinea pig Alexa 488 | Jackson Immunoresearch | 1:100 | |
| Horse-anti-rabbit-biotin | Vector Laboratories | 1:217 | |
| Horse-anti-mouse-biotin | Vector Laboratories | 1:217 | |
| Horse-anti-goat-biotin | Vector Laboratories | 1:217 | |
| Bisbenzimidine | Sigma | 1:2000 | |
| Mouse-Ig-Blocking Reagent (M.O.M.) | Vector Laboratories | 3% | |
| Normal horse serum | Vector Laboratories | 1%, 10% | |
| Streptavidin-Al488 | Vector Laboratories | 1:100 | |
| Streptavidin-Cy3 | Vector Laboratories | 1:1000 | |
1 Antibody reference according to the Resource Identification Initiative at https://scicrunch.org/resources. 2 Antibody activity fades after several months at −80 °C.