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SLC35F2-dependent growth in Cal29 and T24 bladder cancer cells. Knockdown of SLC35F2 by siRNA leads to a decrease in mRNA expression (n = 4) (A) as well as protein expression (B) (for full image, see Supplementary Figure S2) in Cal29 and T24. Cell growth is significantly reduced in SLC35F2 knockdown cells, analysed by crystal violet staining assay (n = 4) (C). Spheroid growth of T24 is also significantly reduced after six days (n = 6) (D). * p < 0.05 Mann–Whitney-U test.
