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. 2021 Jan 22;9:24. doi: 10.1186/s40168-020-00974-y

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Clustering of samples based on the Bray–Curtis dissimilarity of amplicon sequence variant composition generated by averaging the values for the 11 most dominant lineages. The matrices for individual lineages are provided in Fig. S3. Sample names follow the abbreviations shown in Fig. 1, with a suffix indicating the water layer: e, epilimnion; h, hypolimnion. The temporal replicate collected in Lake Biwa in 2010 is abbreviated “BI10.” Five clusters were identified, grouping samples from a Hokkaido, b Europe, c Honshu and Kyushu epilimnia, d Honshu and Kyushu hypolimnia, and e Lake Inawashiro. The total number of reads assigned to each sample is shown by the bar graph on the left (the original data is available in Supplementary Dataset)