Figure 3.

Inhibitory effect of isookanin on NO and PGE₂ production in LPS-induced RAW 267.4 cells. Cells were pretreated with the indicated concentrations of isookanin or positive control for 2 h, before incubation with LPS (100 ng/mL) for 24 h. (A) Cell viability was measured using the WST-1 assay. (B) The levels of NO in the culture medium were measured using the Griess reaction. (C) The levels of PGE₂ in the culture medium were measured using an enzyme-linked immunosorbent assay (ELISA) kit. The results are mean ± standard deviation (SD) (n = 3). # p < 0.01 vs. LPS-untreated control. ** p < 0.01, and *** p < 0.001 vs. LPS-treated control.