Table 3.
Upstream regulators predicted to be activated (orange) or inhibited (blue) in wooden breast (WB) myopathy compared to normal muscle based on differentially expressed downstream target molecules in commercial broilers.
| Upstream Regulator | Protein Name | Activation z-Score | p-Value of Overlap | Differentially Expressed Downstream Target Proteins 1 |
|---|---|---|---|---|
| ANGPT2 | Angiopoeitin 2 | 3.08 | 7.41 × 10−9 | CALR, CRYAB, HSP90AA1, HSPA2, HSPA5, P4HB, PDIA3, PDIA6, POSTN, RPS6 |
| EGFR | Epidermal growth factor | 2.62 | 1.38 × 10−3 | CRYAB, HNRNPH1, HSP90B1, HSPA5, POSTN, PPIA, VIM |
| XBP1 | X box binding protein 1 | 2.41 | 8.79 × 10−3 | CALR, HSP90B1, HSPA5, PDIA3, PDIA4, PDIA6 |
| TGFB1 | Transforming growth factor beta 1 | 2.21 | 1.95 × 10−6 | AK1, ANXA2, CAPNS1, COL7A1, CTSB, DES, EEF1A1, FLNA, FSCN1, GATD3A/GATD3B, HNT1, HNRNPH1, HSP90A1, HAPA5, IARS, IGHM, LDHA, PGK1, PLS3, POSTN, RAB1A, RACK1, RHOA, VIM, YWHAE |
| NFE2L2 | Nuclear factor (erythroid-derived 2)-like 2 | 2.18 | 4.3 × 10−9 | ARF1, CBR1, DSTN, HSP90AA1, HSP90B1, ME1, PDIA3, PDIA4, PDIA6, PGD, RACK1, RAN, RPL18, RPLPO, TPI1 |
| RICTOR | Regulatory-associated protein Independent of mTOR complex 2 | −3.61 | 2.06 × 10−9 | PSMC2, RPL11, RPL18, RPL4, RPL6, RPLP0, RPS11, RPS2, RPS24, RPS3 |
| PPARGC1A | Peroxisome proliferator activator receptor gamma-coactivator 1 α | −2.35 | 1.92 × 10−4 | AK1, C3, CS, GOT2, LDHA, ME1, PGAM1 |
| AHR | Aryl hydrocarbon receptor | −2.22 | 2.02 × 10−3 | ABCB6, CBR1, GOT1, HSP90AA1, HSPA5, IGHM, VIM |
| ARNT | Aryl hydrocarbon receptor nuclear translocator | −2.20 | 3.35 × 10−6 | ALDOC, GAPDH, IGHM, LDHA, PGK1, TPI1, VIM |
| ESRRG (NR3B3) | Estrogen related receptor gamma | −2.17 | 1.89 × 10−6 | ALDOC, GAPDH, LDHA, PKM, TPI1 |
1 Target molecules in plain red type were up-regulated (p < 0.05, >1.3-fold difference) in WB myopathy compared to normal tissue. Target molecules in bold green and underlined type were down-regulated (p < 0.05, <−1.3-fold difference) in WB myopathy compared to normal tissue. ANGPT2: Angiopoeitin 2; EGFR: epidermal growth factor; XBP1: X box binding protein; TGFB1: transforming growth factor beta 1; NFE2L2: nuclear factor (erythroid-derived 2)-like 2.