Figure 4.

CETP inhibition decreases NADPH oxidase activation. Representative microscopic images of HAEC stained with (A) p47^phox (B) NOX2 (gp91^phox) and DAPI. p47^phox and NOX2 (green) are localized to the plasma membrane, stained with wheat germ agglutinin (WGA-red). After CETP inhibition, p47^phox and NOX2 co-localize with cytoplasmic aggregates of endogenous actin, detectable with phalloidin labeling. Upper panel 10X magnification and bottom 20X. Scale bar, 10 μm. (C) Western blotting of p47^phox and NOX2 (gp91^phox) in the plasma membrane fraction of HAEC normalized by the L-type calcium channel protein (n = 3, * p < 0.05). HAECs, human aortic endothelial cells. CETP, cholesteryl ester transfer protein. NS, non-silenced. siCETP, silenced CETP.