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. 2021 Jan 7;11(1):69. doi: 10.3390/biom11010069

Figure 7.

Figure 7

CETP inhibition reduces adhesion molecules expression and monocyte adhesion in endothelial cells. (A) Fluorescently stained THP-1 cells adhered to the monolayer of HAECs transfected with siCETP or control scrambled siRNA. The average number of THP-1 cells adhered on the monolayer of HAECs in a field with the same size was quantified and results are expressed as Mean ± SEM (n = 3), * p < 0.05. Protein expression and mRNA levels of adhesion molecules in HAECs: (B) VCAM-1 immunoblot and qPCR analysis of CD106 mRNA, (C) ICAM-1 immunoblot and qPCR analysis of CD54 mRNA. Protein levels were normalized to Tubulin or HSP90 (* p < 0.05). (D,E) qPCR analysis of TNFA and CCL2 mRNA in HAECs transfected with siCETP or control scrambled siRNA. Results are expressed as Mean ± SEM (n = 3), * p < 0.05. HAECs, human aortic endothelial cells. CETP, cholesteryl ester transfer protein. NS, non-silenced. siCETP, silenced CETP.