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Detection of NS6 protein expression in the PDCoV target tissues in vivo. Representative immunohistochemical (IHC) staining in the jejunum and ileum from PDCoV-infected or mock-infected piglets. Samples were stained with pre-immune mouse serum (1:200), mouse anti-NS6 antiserum (1:200) or anti-M (1:100) antibodies. Black arrows indicate positive antigens; magnification = 300 × .
