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. 2021 Jan 7;13(1):75. doi: 10.3390/v13010075

Figure 6.

Figure 6

Sar55 replication enhanced in ORF4−expressing Huh7 cell lines treated with Tunicamycin. (A) Immunofluorescence detection of HEV ORF2 antigen in methanol fixed ORF4+ Huh7 cell treated with 15 µg/mL tunicamycin after 5 dpt with Alexa fluor 594 (red) and DAPI (blue). (B) Flow cytometry quantification of ORF4+ Huh7 cells transfected with the capped RNA transcripts of the HEV gt3 (P6) strain and HEV gt1 (Sar55) strain, performed at 5 dpt. Samples were fixed in methanol and probed with rabbit anti-ORF2 followed by goat anti-rabbit PE antibodies. Each bar (mean ± SEM) represents separate transfections stained in parallel and display the mean of three independent biological experiments with nine replicates per sample (** p < 0.01, t-test). Statistically significant difference exists between Sar55 transfected, endoplasmic stressor (tunicamycin) treated ORF4+ cell lines and untreated cell lines.