Table 1.
Effects of oleic acid, homovanillyl alcohol, and hydroxytyrosol treatment on metabolic gene expression in A375 and MNT1 cells after 48 h of exposure by RT-qPCR analysis.
| A375 Melanoma Cells | MNT1 Melanoma Cells | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Oleic Acid | Homovanillyl Alcohol | Hydroxytyrosol | Oleic Acid | Homovanillyl Alcohol | Hydroxytyrosol | |||||||
| Gene | 100 µM | 200 µM | 100 µM | 200 µM | 100 µM | 200 µM | 100 µM | 200 µM | 100 µM | 200 µM | 100 µM | 200 µM |
| SNAT1 | nss | nss | nss | nss | nss | p = 0.017 | Nss | nss | Nss | nss | nss | nss |
| SNAT2 | nss | nss | nss | nss | nss | nss | Nss | nss | Nss | nss | nss | nss |
| GLS1 | nss | nss | nss | nss | nss | nss | Nss | nss | Nss | nss | nss | nss |
| LDHA | nss | nss | nss | nss | nss | nss | p = 0.043 | nss | Nss | nss | p = 0.037 | p = 0.012 |
| LDHB | nss | nss | nss | nss | nss | nss | p = 0.039 | nss | nss | nss | nss | nss |
| LDHC | nss | nss | nss | nss | nss | nss | p = 0.042 | nss | nss | nss | nss | p = 0.042 |
| MCT1 | nss | nss | nss | nss | nss | nss | Nss | nss | nss | nss | nss | nss |
| MCT4 | nss | nss | nss | nss | nss | nss | Nss | nss | nss | nss | nss | nss |
| GLUL | nss | nss | nss | nss | nss | nss | Nss | nss | nss | nss | nss | nss |
| G6PD | nss | nss | nss | nss | nss | nss | Nss | nss | nss | nss | nss | nss |
| xCT | nss | nss | p = 0.047 | nss | nss | p = 0.009 | Nss | nss | nss | nss | nss | nss |
| EAAT3 | nss | nss | nss | nss | nss | p = 0.008 | Nss | nss | nss | nss | nss | nss |
nss—Not statistically significant. Green—genes upregulated. Red—genes downregulated.