Figure 8.

The gut metabolome is significantly impacted by genistein (GEN) supplementation. The level of select fecal metabolites that were significantly increased by GEN supplementation, compared with non-supplemented state, regardless of maternal dietary exposure: (A) phloretin, (B) ribothymidine, and (C) estradiol. Abundance of some metabolites were similarly reduced by GEN supplementation in the control and HFD offspring: (D) trigonelline; and some were reduced only in the HFD offspring (E): tyramine. (F) Additional select pathways which were significantly affected by GEN supplementation only in the HFD offspring. The selected pathways were: A, spermine biosynthesis; B, spermidine biosynthesis; C, spermine and spermidine degradation; E, aspirin triggered resolvin E biosynthesis, the yellow line is designed to highlight the significance; F, aspirin triggered resolvin D biosynthesis; and G, resolvin D biosynthesis. Significance was defined as -log₁₀ p value. The blue dashed line represented significance of p = 0.01. The Dysregulation ratio was defined as the number of dysregulated to the number of total metabolites detected in the dataset that were assigned to a given pathway. (G) The linear correlation between the relative abundance of the genus Lactococcus and the fecal trigonelline level.