Figure 1.

Generation of endothelial cell-specific deletion of CR6-interacting factor1 (CRIF1) in mice. (a) Schematic diagram showing endothelial deletion of CRIF1 gene target strategy. The numbers indicate exons of CRIF1, and the triangles show the loxp sites. (b) Representative images of PCR genotyping for CRIF1^loxp and Cre primers are shown. WT: flox/flox/Cre– and flox/flox+/Cre–; Hetero: flox/flox+/Cre; CRIF1: flox/flox/Cre. (c) mRNA expression was examined by qPCR in the cardiac endothelial cells of mice. (d) Western blotting analysis of CRIF1 in the endothelial and non-endothelial cells isolated from the hearts of mice. CRIF1 protein levels were quantified by densitometric analysis. The data are presented as means ± SEM of at least three independent experiments (n = 5 mice in each group; ns: not significant; * p < 0.05 vs. ff/– group).