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. 2021 Jan 12;10(1):135. doi: 10.3390/cells10010135

Figure 3.

Figure 3

Knockdown of SOX2 in radioresistant colorectal cancer cells attenuated the induction of colorectal CSCs after irradiation. (A) Immunoblotting for the expression of CSC-related proteins on day 2 after radiation (10 Gy) in colorectal cancer cells as indicated. (B) siRNA-mediated SOX2 knockdown in cells was identified by western blotting (left) and CD44+ cell population (middle), or apoptotic cells were analyzed by flow cytometry (right). All experiments were performed with the SOX2 siRNA-transfected HCT116 and DLD1 cells on day 2 after radiation (10 Gy). Data are shown as mean ± SD (n = 3). * p < 0.05 compared si-Cont + IR to si-SOX2 + IR. (C) The images of migration and invasion on day 2 after radiation (10 Gy) of the SOX2 siRNA-transfected HCT116 and DLD1 cells were quantified. Bars indicate measurements of migration and invasion. * p < 0.05 compared si-Cont + IR to si-SOX2 + IR. (D) Tumoursphere-formation assay was performed to evaluate self-renewal ability of CSCs in SOX2 siRNA-transfected cells. Indicated cells were seeded in a non-adherent culture condition. After culturing for 7 days, the number of tumoursphere cells (>100 μm diameter) was quantified. Data are shown as mean ± SD (n = 3). * p < 0.05 versus si-Cont. IR: irradiation, Si-Cont: control siRNA, si-SOX2: SOX2 siRNA, CSCs: cancer stem-like cells.