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Intracellular locations of E. coli MG1655 (red) in A. polyphaga cysts at 96 h of co-culture. Many of the cysts did not contain any E. coli MG1655 cells, which indicate that the digestion may depend on the intracellular load of the bacterial cells and encystation time. Releasing of vesicle during encystation and vesicle-containing E. coli MG1655 after 48 h of co-culture (Right image).
