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. 2021 Jan 15;10(1):1868122. doi: 10.1080/2162402X.2020.1868122

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© 2021 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 7. — IL-12 blocking counteracted the increase of TILs derived from B. breve treatment. a. Representative H&E staining images of tumor tissues were shown in the left panel, and the expressions of CD3, CD4, and CD8 in tumor tissues were detected by immunohistochemical staining assay (upper; scale bar: 50 μm). The quantitative analysis was performed by ImageJ software and the number of positive cells were presented in scatter plots (lower). b. Percentage of CD3⁺, CD4⁺, and CD8⁺ T cells within the tumor of C3H/HeN mice from each group were assessed by flow cytometry and the statistical analysis was shown in scatter plots. Each dot represents one mouse (n = 5/group). All these data were analyzed with one-way ANOVA and presented as mean ± SD (*P < .05; **P < .01; ***P < .001; ****P < .0001)