REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse anti-V5	Invitrogen	Cat# R960-25, Lot 1949337; RRID: AB_2556564
Rabbit anti-mCherry	Novus Biologicals	Cat# NBP2-25157, Lot 102816; RRID: AB_2753204
Rat anti-HA	Roche	Cat# 11867423001, Lot 27573500; RRID: AB_390918
Donkey anti-mouse IgG Alexa Fluor 488	Jackson ImmunoResearch	Cat# 715-545-151; RRID: AB_2341099
Donkey anti-rat IgG Alexa Fluor 647	Jackson ImmunoResearch	Cat# 712-605-153; RRID: AB_2340694
Donkey anti-rabbit IgG Rhodamine RedTM-X (RRX)	Jackson ImmunoResearch	Cat# 711-295-152; RRID: AB_2340613
Chicken anti-GFP	Abcam	Cat# 13970; RRID: AB_300798
Rabbit anti-dsRed	Clontech	Cat# 632496; RRID: AB_10013483
Goat anti-chicken Alexa Fluor 488	Invitrogen	Cat# A11039; RRID: AB_2534096
Goat anti-rabbit Alexa Fluor 568	Invitrogen	Cat# A11011; RRID: AB_143157
Chemicals, Peptides, and Recombinant Proteins
All-trans-retinal	Toronto Research Chemicals	Cat# R240000
Experimental Models: Organisms/Strains
Drosophila: w;; attP2 (empty insertion site)	Pfeiffer et al., 2008	N/A
Drosophila: w; iav-GAL4 in VK00014	Bloomington Drosophila Stock Center	Derived from: BDSC: 36360
Drosophila: w;; iav-GAL4	Bloomington Drosophila Stock Center	BDSC: 52273
Drosophila: w; ppk-LexA in attP40	Vogelstein et al., 2014	N/A
Drosophila: w; R26F05-LexA in attP40	Pfeiffer et al., 2010	BDSC: 54702
Drosophila: w; R61D08-LexAp65 in JK22C	Pfeiffer et al., 2010	N/A
Drosophila: w;; R61D08-GAL4 in attP2	Pfeiffer et al., 2008	BDSC: 39272
Drosophila: w;; R72F11-GAL4 in attP2	Pfeiffer et al., 2008	BDSC: 39786
Drosophila: w; R72F11-LexAp65 in JK22C	Pfeiffer et al., 2010	N/A
Drosophila: w;; ppk-LexA in attP2	Vogelstein et al., 2014	N/A
Drosophila: w;; ppk-QF2	Bloomington Drosophila Stock Center	BDSC: 66475
Drosophila: w;; 165-GAL4	Gift from W. Grueber	N/A
Drosophila w; mhc[1]	Gift from N. Brown	N/A
Drosophila: w; 13XLexAop2-IVS-myr::GFP in su(Hw)attP5	Pfeiffer et al., 2012	N/A
Drosophila: w;; UAS-IVS-myr::tdTomato in attP2	Pfeiffer et al., 2010	BDSC: 32221
Drosophila: w, QUAS-syn21-CsChrimson tdTomato_tr p10 in attP18	This study	N/A
Drosophila: w; 13XLexAop2-CsChrimson-tdTomato in attP40	Gift from V. Jayaraman	N/A
Drosophila: w;; 13XLexAop2-CsChrimsontdTomato in VK00005	Gift from V. Jayaraman	BDSC: 82183
Drosophila: w, 10XUAS-Syn21-Chrimson88-tdT-3.1 in attP18	Gift from A. Wong	N/A
Drosophila: w;; 20xUAS-IVS-GCaMP6s 15.641 in attP2	Gift from V. Jayaraman	N/A
Drosophila: w;; pJFRC97-20XUAS-IVS-GCaMP3-p10 in attP2	Pfeiffer et al., 2012	N/A
Drosophila: w, LexAop2-Syn21-opGCaMP6s in su(Hw)attP8	Gift from Allan Wong	N/A
Drosophila: w; pSW922[260b] (LexAop-TNT)	Gift from B. Dickson	N/A
Drosophila: w; UAS-TNT-E	Sweeney et al., 1995	N/A
Drosophila: w; 13XLexAop2-IVS-Syn21-Shibire-ts1-p10 in su(Hw)attP5	Pfeiffer et al., 2012	N/A
Drosophila: w;; 20XUAS-TTS-Shibire-ts1-p10 in VK00005	Pfeiffer et al., 2012	BDSC: 66600
Drosophila: w;; UAS-Kir 2.1	Bloomington Drosophila Stock Center	BDSC: 6595
Drosophila: w;; pJFRC26-13XLexAop2-IVS-dTrpA1-WPRE in VK00005	Pfeiffer et al., 2010	N/A
Drosophila: w;; UAS-FraRobo	Bashaw and Goodman, 1999	N/A
Drosophila: w, 10xUAS-IVS-myr::smGdP-HA in attP18	Bloomington Drosophila Stock Center	BDSC: 64092
Drosophila: w, 13xLexAop2-IVS-myr::smGdP-V5 in su(Hw)attP8	Bloomington Drosophila Stock Center	BDSC: 64092
Drosophila: w; UAS-bruchpilot (short)-mstraw	Gift from S. Sigrist	N/A
Drosophila: w; UAS-LacZ	Bloomington Drosophila Stock Center	BDSC: 8530
Drosophila: w; UAS-robo-2::HA	Bloomington Drosophila Stock Center	BDSC: 66886
Drosophila: w; UAS-unc-5::HA	Gift from B. Dickson	N/A
Software and Algorithms
CATMAID	Saalfeld et al., 2009; Schneider-Mizell et al., 2016	https://www.catmaid.org
R	R Core Team, 2015	https://www.r-project.org/
FIJI	Schindelin et al., 2012	https://imagej.net/Fiji
LARA package	Denisov et al., 2013; Ohyama et al., 2013	https://sourceforge.net/projects/salam-hhmi
Multi Worm Tracker	Swierczek et al., 2011	https://sourceforge.net/projects/mwt