We rechecked this paper and realized that some data in Figures 6F and 7C were unintentionally misplaced. We found the inadvertent errors occurred during figure layout. We have accordingly corrected and replaced the image in Figures 6F and 7C. This correction has not changed the description, interpretation, or the original conclusions of the manuscript. The authors apologize for any inconvenience that caused by this unintentional misplacement.
Figure 6.
PKM2 silencing mainly abolished PTBP1-induced proliferation in ccRCC cells. A and B. Verification the efficiency of PTBP1 overexpression and PKM2 knockdown in 769P and ACHN cells by RT-qPCR. C. Verification of PKM2 knockdown efficiency by western blots. D. MTT assay evaluation of influence of PKM2 knockdown on 769P and ACHN cell viability. E. Colony formation assay evaluation the effect of PKM2 knockdown on 769P and ACHN cells. F. Flow cytometry analysis of 769P and ACHN cells transfected with control or PKM2 siRNA for 72 h. The percentages (%) of cell populations at different stages of the cell cycle are listed in the panels. All histograms show the percentage (%) (means ± SD) of cell populations from three independent experiments. *P < 0.05, **P < 0.01.
Figure 7.
Knockdown of PKM2 abrogated the promotion of migration and invasion induced by PTBP1 overexpression in ccRCC cells. A and C. Representative pictures showing migrated and invasive cells under microscope (200×). B and D. All histograms represent the number (mean ± SD) of migrated or invasive cells from three independent experiments. Statistical significance was calculated using the ANOVA. *P < 0.05, **P < 0.01.