TABLE 3.

Environmental sampling: data set of collected samples and testing^a

Classification		No. of samples in data set (n = 94)	Description	Nucleic acid testing
Surface source	Sample
				SARS-CoV-2 (RT-qPCR)	Biofluid (qPCR)	Microbiota (NGS)
Indoor	Hospital	49	Floor (n = 7)	+	+	+
			Bedside table (n = 3)	+	+	+
			Door handle (n = 3)	+	+	+
			Call button (n = 1)	+	+	+
			Table (n = 1)	+	+	+
			Chair (n = 2)	+	+	+
			Back of the bed (n = 3)	+	+	+
			Side of bed (n = 4)	+	+	+
			Bottom of the bed (n = 1)	+	+	+
			Wall behind the bed head (n = 1)	+	+	+
			Bed sheets (n = 1)	+	+	+
			Pillow (n = 2)	+	+	+
			Stethoscope (n = 1)	+	+	+
			Wheelchair head (n = 1)	+	+	+
			Toilet board (n = 1)	+	+	+
			Toilet flush button (n = 1)	+	+	+
			Sink faucet (n = 1)	+	+	+
			Air circulation system (15)	+	−	−
	Public building	25	Door handle (n = 2)	±	+	+
			Toilet (n = 2)	+	+	+
			Pews (n = 4)	−	+	+
			Floor (n = 6)	±	+	+
			Toilet wall tiles (n = 3)	±	+	+
			Office phone (n = 1)	+	+	+
			Computer keyboard (n = 2)	+	+	+
			Air circulation system (n = 5)	−	+	+
Outdoor		16	Handrail (n = 1)	+	+	+
			Grip shared e-scouter (n = 1)	+	+	+
			Bus stop bench (n = 1)	+	+	+
			Coffee dispenser button (n = 1)	+	+	+
			External door handle (n = 2)	±	+	+
			Playground (n = 10)	−	±	+
Human		4	Droplet biofluid (n = 4)	±	+	+

^aIndoor surfaces were sampled from different hospitals (n = 3), buildings of public use (one office, one fast food restaurant, and one church), outdoor areas (n = 16), nose-oropharyngeal secretions (n = 4) and tested for the presence of SARS-CoV-2 by quantitative reverse transcription real-time PCR (RT-qPCR) and for anthropic contamination by testing the presence of microbiota traces of biological fluids (nose, saliva, Feces), by quantitative real-time PCR (qPCR) and/or next generation sequencing (NGS). For each type of test, analysis was performed in all (+), none (−), or some (±) of the collected samples.