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Kinetics of crotonyl-CoA hydratase (A) and 3-hydroxypropionyl-CoA dehydratase (B) reactions in extracts of autotrophically grown M. sedula. The determined Vmax and Km values were: (A) 15.7 ± 0.6 U mg−1 protein and 23 ± 3 μM for crotonyl-CoA and (B) 1.18 ± 0.02 U mg−1 protein and 109 ± 5 μM for 3-hydroxypropionyl-CoA.
