BMP signaling blockade changes the frequencies of myeloid subsets in the peripheral immune system. Freshly isolated splenocytes were stained for flow cytometry analysis to quantify the frequencies of B-cell subsets, T-cell subsets, and myeloid subsets in the periphery in vehicle- and DMH1-treated mice. Regarding myeloid subsets, total CD11b+ myeloid cells and F4/80+ macrophages were analyzed in the first experiment, and in the second experiment, the frequencies of different DC subsets (lymphoid, myeloid and plasmacytoid) and the macrophage type 2 (M2) phenotype were assessed. After excluding doublets and dead cells, the indicated gating strategies were followed to determine the frequencies of (a) myeloid cells (CD11bhigh events) and macrophages (CD11b+F4/80+ events), (b) lymphoid DCs (CD11chighCD8a+ events in the B220−CD11b+ population), myeloid DCs (CD11chighCD8a− events in the B220−CD11b+ population) and pDCs (CD11cintCD317+ events in the B220+CD11b− population), and (c) M2 macrophages (Ly6C−CD206+ events in the Ly6G−CD11b+F4/80+ population). (d) Graphs represent the percentages of the studied populations. Data are represented as the mean ± standard error of the mean (SEM). The statistical analysis was performed with Student’s t test. DMH1 = dorsomorphin homologue 1; M2 = macrophage type 2