Extended Data Fig.9. Linker histone incorporation reduces interactivity of chromatin fiber.

a, Representative equilibrated configurations of 50-nucleosome chromatin fibers obtained in silico in absence of H1 and in presence of 0.25, 0.5, 0.75, and 1 H1 per nucleosome - H1C (left) and H1E (right). Fiber contour (in red) on which the H1 mean positions are shown is shown on the top right of each model. Color key shows DNA, linker histone, H2A, H2B, H3, and H4 tails. b-c, Contact maps for the 1000-configuration ensembles obtained from left to right, in absence of H1 and in presence of 0.25, 0.50, 0.75, and 1 H1 per nucleosome, H1C in (b) and H1E in (c). d, The nucleosome/nucleosome interaction patterns, or a one-dimensional decomposition of each contact map shown in (b) and (c), indicate the dominant zigzag pattern of the fiber (i+/−2) and increase of long-range interactions as the H1C (left) or H1E (right) density decreases. e, Packing ratio calculated as the number of nucleosomes contained in 11 nm of fiber for systems without H1 and in presence of 0.25, 0.50, 0.75, and 1 H1 per nucleosome (left, H1C and right, H1E). n=1000 chromatin ensemble configurations for each H1 per nucleosome ratio. Ordinary one-way ANOVA for multiple comparisons, ****P<0.0001. Boxplot center represents median, bounds of box are 1^stand 3^rdquartile and whiskers extend out 1.5*interquartile range from the box. f, Volume of chromatin fibers calculated assuming a cylindrical shape for systems with no H1 and in presence of 0.25, 0.5, 0.75, and 1 H1 molecule per nucleosome (left, H1C and right, H1E), n=1000 chromatin ensemble configurations for each H1 per nucleosome ratio. Ordinary one-way ANOVA for multiple comparisons, ****P<0.0001. Boxplot center represents median, bounds of box are 1^st and 3^rd quartile and whiskers extend out 1.5*interquartile range from the box.