Figure 6. Depletion of TAMs in Atoh1-SmoM2 mice leads to an increase of pro-inflammatory T cells.

(A) Schematic representation of experimental design. (B–D) Number of CD8⁺ T cells (B), CD4⁺ T cells (C), and CD45⁺ immune cells (D) per milligram (mg) of tumor in control (CTRL)- and PLX-treated tumors. (E) Percentage of Granzyme B⁺ (Gzmb) CD107⁺CD8⁺ T cells of total CD8⁺ T cells. (F) Number of Gzmb⁺ CD107⁺CD8⁺ T cells per mg of tumor. (G–I) Representative flow cytometry panels from the CD45^hi TCRβ⁺ CD8⁺ population (G) showing the proportion (H) and the number (I) of IFNγ⁺CD8⁺ T cells. (J–L) Representative flow cytometry panels from the CD45^hiTCRβ⁺CD4⁺ population (J) showing the proportion (K) and the number (L) of IFNγ⁺CD4⁺ T cells. Mean ± SD. Significance was determined using unpaired t test for (B–F), (H–I) and (K–L). **p<0.01.