Skip to main content
. Author manuscript; available in PMC: 2022 Mar 1.
Published in final edited form as: Bone. 2020 Dec 16;144:115819. doi: 10.1016/j.bone.2020.115819

Figure 2: GATA4 regulates both osteogenesis and adipogenesis in vitro.

Figure 2:

Bone marrow MSCs from WT and Gata4 Prx-cKO were differentiated in osteogenic media for two weeks. (A) RNA was obtained and qPCR was performed for Gata4 and normalized to actin mRNA. (B) Cells were fixed and stained for mineralization using Alizarin Red. (C) Alizarin Red from part B was eluted and the mineral content measured at an OD of 570 nm. (D) qPCR was performed for Runx2 and (E) osteocalcin (OCN, BGLAP). (F) Bone marrow MSCs were also differentiated for two weeks in adipogenic media and qPCR was performed for Gata4 normalized to actin mRNA. (G) Adipocytes were stained using Oil Red O and (H) the percentage of fat droplets were calculated using ImageJ. (I) PPARγ and (J) Adiponectin expression was evaluated in the WT and Prx-cKO adipocytes. Student’s t test; * P < 0.05; *** P < 0.001 compared with wild-type. OD = optical density.