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. Author manuscript; available in PMC: 2022 Feb 1.
Published in final edited form as: Hypertension. 2020 Nov 30;77(2):420–431. doi: 10.1161/HYPERTENSIONAHA.120.15793

Figure 5: Effect of ICV TRV027 on water intake in absence of DOCA.

Figure 5:

A. The effect of TRV027 on water intake in the absence of DOCA was measured. Three groups of mice underwent 2-bottle choice experiment: 1) sham group received intracerebroventricular (ICV) infusion of vehicle (aCSF), 2) ICV Ang II and 3) ICV TRV027. Total daily water intake (left) and side-bias (right) was measured when mice were presented with 2 burettes filled with tap water. Data are expressed as mean±SEM. Data were analyzed by one-way ANOVA with Tukey’s multiple comparisons procedure. *p<0.05 compared with vehicle. #p<0.05 compared with Ang-II. aCSF(n=6), Ang II (n=7), TRV027(n=8). B. The same three experimental groups were presented with a choice of tap water vs different concentrations of saline. Mice were then presented with a choice of water vs 0.15M NaCl (top), water vs 0.30M NaCl (middle) and water vs 0.45M NaCl (bottom). Panel 1 (left): daily water intake; Panel 2 (center): daily saline intake; Panel 3 (right): daily fluid intake calculated as daily water intake plus daily saline intake. Data are expressed as mean±SEM. Data were analyzed by one-way ANOVA with Tukey’s multiple comparisons procedure. *p<0.05 compared with vehicle. #p<0.05 compared with Ang-II. aCSF(n=6), Ang II (n=7), and TRV027(n=7)