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. Author manuscript; available in PMC: 2022 Jan 12.
Published in final edited form as: Circulation. 2020 Oct 27;143(2):145–159. doi: 10.1161/CIRCULATIONAHA.120.049813

Figure 4. 12,13-diHOME increases function and respiration in isolated cardiomyocytes.

Figure 4.

(A) Peak shortening, (B) Ca2+ transient, (C) maximal velocity of shortening, and (D) maximal velocity of relengthening in isolated cardiomyocytes ±12,13-diHOME. Data are mean ± S.E.M (n=4/group; 10-12 myocytes per mouse). Asterisks represent difference compared to vehicle (**P<0.01; ***P<0.001). (E) Fatty acid uptake in cardiomyocytes constitutively expressing firefly luciferase that were treated with either 12,13-diHOME or vehicle, as measured by luciferase activity using 10 uM FFA-SS-Luc. Data are mean ± S.E.M (n=9 technical replicate wells per group. Asterisks represent difference compared to vehicle (**P<0.01). (F) Bioenergetic profile of cardiomyocytes treated with 12,13-diHOME or vehicle. (G) Basal OCR, (H) maximal respiration, and (I) non-mitochondrial respiration were measured. Data are mean ± S.E.M (n=5/group). Asterisks represent differences compared to vehicle (*P<0.05; ***P<0.001). (J) Bioenergetic profile of cardiomyocytes treated with 12,13-diHOME or vehicle with or without BDM. (K) Basal OCR, (L) maximal respiration, and (M) non-mitochondrial respiration were measured. Data are mean ± S.E.M (n=5/group). Asterisks represent differences compared to vehicle (*P<0.05; ***P<0.001), compared to vehicle + BDM (##P<0.01; ###P<0.001), or compared to 12,13-diHOME + BDM ($$$P<0.001). Unpaired two-tailed Student’s t-test was used for A, B, C, D, G, H, and I. Two-way ANOVA was used for E, F and J with Tukey’s multiple comparisons tests; one-way ANOVA was used for K, L, and M with Tukey’s multiple comparisons tests.