TABLE 1.

Known heme uptake, regulatory, and synthesis proteins in P. aeruginosa

Gene	Locus no.a	Enzyme or function	No. of Tn inserts in original library	Reference(s)
Uptake
hxuA	PA1302	Heme outer membrane receptor		148
hxuR	PA1301	Anti-sigma factor		70, 148
hxuI	PA1300	ECF sigma factor		70, 148
hasD	PA3406	Transport protein		149
hasA	PA3407	Extracellular heme binding (hemophore)		149
hasR	PA3408	Heme outer membrane receptor (sensing)	1	149
hasE	PA3405	Membrane fusion protein		149
hasF	PA3404	Outer membrane protein		149
hasS	PA3409	Anti-sigma factor		149
hasI	PA4310	ECF sigma factor		149
phuR	PA4710	Major heme outer membrane receptor	5	149
phuU	PA4707	Inner membrane heme transporter	1	149
phuV	PA4706	Inner membrane heme transporter	1	149
phuW	PA4705	Possible PhuR auxiliary protein		149
phuS	PA4709	Heme trafficking	1	149
phuT	PA4708	Heme trafficking		149
Catabolism
hemO	PA0672	Major heme oxygenase	1	10
bphO	PA4116	Phytochrome heme oxygenase	1	150
Regulation
dnr	PA0527	NO responsive transcription factor	1	56
anr	PA1544	O2 responsive transcription factor		56
prrF1/2	NAb	Iron responsive sRNA		35
Synthesis (of HemB)
gltX	PA3134	Glutamyl-tRNA synthetase		151
hemA		ALA synthase	NA	1
hemA	PA4666	Glu-tRNA reductase		152
hemL1	PA3977	Glutamate-1-semialdehyde 2,1-aminomutase		1
hemL2	PA4088	Glutamate-1-semialdehyde 2,1-aminomutase		1
hemL3	PA5523	Glutamate-1-semialdehyde 2,1-aminomutase	2, 1c	1
hemB	PA5243	PBG synthase		1
hemC	PA5260	HMB synthase		1
hemD	PA5259	URO synthase		1
hemE	PA5034	URO decarboxylase		1
hemF	PA0024	Coproporphyrinogen decarboxylase	1	1
hemN/Z	PA1546	O2-independent coproporphyrinogen III oxidase	2	1
hemG		Protoporphyrinogen dehydrogenase	NA	1
hemJd	PA0661	Protoporphyrinogen dehydrogenase	1	1
hemK	PA4664	Methyltransferase	1	1
hemY	PA5257	Protoporphyrinogen oxygenase		153
hemH	PA4655	Protoporphyrin ferrochelatase		1
hemX	PA5258	Membrane heme biosynthesis regulatory protein		153
HemD1 synthesis
nirF	PA0516	HemD1 biosynthesis	2	154
nirL	PA0514	HemD1 biosynthesis		154
nirJ	PA0511	HemD1 biosynthesis	1	154
nirE	PA0510	Uroporphyrin III c-methyltransferase	1	154
Siroheme
cysG	PA2611	Siroheme synthase		155
cobA	PA1778	Uroporphyrin III methyltransferase	1	155
Colbamin (B12)
cobI	PA2904	Cobalt-factor-2 C20-methyltransferase		156
cobG	PA2906	Precorrin-3B synthase	1	156
cobJ	PA2903	Precorrin-3B C17-methyltransferase		156
cobM	PA2948	Cobalt-precorrin-4 C11-methyltransferase		156
cobF		Precorrin-6A synthase	NA	156
cobK	PA2909	Cobalt-precorrin-6A reductase	1	156
cobH	PA2905	Cobalt-precorrin-8 methylmutase		156
cobB	PA1273	c-Diamide synthase		156
cobN1	PA2944	Cobaltochelatase	3	156
cobN2	PA1923	Cobaltochelatase		156
cobO	PA1272	Cob(I)alamin adenosyltransferase	1c	156
cobQ	PA1277	Adenosylcobyric acid synthase		156
cobC	PA1276	Threonine-phosphate decarboxylase		156
cobD	PA1275	Adenosylcobinamide-phosphate synthase		156
cobP	PA1278	Adenosylcobinamide kinase		156
cobV	PA1281	Adenosylcobinamide-GDP ribazoletransferase		156
Hypothetical	PA1280	Alpha-ribazole phosphatase		156
cobU	PA1279	Nicotinate-nucleotide-dimethylbenzimidazole phosphoribosyltransferase	1	156
cobL	PA2907	Precorrin-6Y C5,15-methyltransferase		156

^aLocus for PAO1 strain.

^bNA, not applicable.

^cTn insertion disrupts predicted promoter.

^dHomology by sequence only.