Figure 1. Comparison between conventional and Detection of Amplification by Releasing of Quenching loop-mediated isothermal amplification.

All reactions were performed using E1 primer targeting SARS-CoV-2 RNA at 10⁴, 10³ 10² and 50 copies, and NTC in triplicate. (A) Conventional LAMP monitored by SYTO-9. (B) Single-plex DARQ LAMP. (C) Duplex DARQ LAMP including both E1 and ACTB primers. (D) ACTB detection from duplex DARQ LAMP. (E) Comparison of SARS-CoV-2 amplification speed in reactions with SYTO-9, single-plex and duplex DARQ LAMP.

DARQ: Detection of Amplification by Releasing of Quenching; LAMP: Loop-mediated isothermal amplification; NTC: No-template control.