Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (TH-Cre Long-Evans rats, male and female) |
LE-Tg(TH-Cre)3.1Deis | Rat Resource and Research Center | RRRC#: 00659; RRID:RGD_10401201 |
|
| Antibody | anti-GFP (Mouse monoclonal) |
Millipore Sigma | Cat. #: MAB3580; RRID:AB_94936 |
(1:1500) |
| Antibody | anti-TH (Rabbit polyclonal) |
Millipore Sigma | Cat. #: AB152; RRID:AB_390204 |
(1:2000) |
| Antibody | Alexa Fluor 488 donkey anti-mouse IgG (Donkey polyclonal) |
Thermo Fisher | Cat. #: A-21202; RRID:AB_141607 |
(1:500) |
| Antibody | Alexa Fluor 555 donkey anti-rabbit IgG (Donkey polyclonal) |
Thermo Fisher | Cat. #: A-31572; RRID:AB_162543 |
(1:500) |
| Recombinant DNA reagent | AAV5-EF1α-DIO-hChR2 (H134R)-EFYP |
UNC Vector Core | ||
| Recombinant DNA reagent | AAV5-EF1α-DIO-eArch3.0-EYFP | UNC Vector Core | ||
| Recombinant DNA reagent | AAV-EF1α-DIO-EYFP | UNC Vector Core | ||
| Software, algorithm | Matlab | Mathworks | RRID:SCR_001622 | |
| Software, algorithm | RStudio | RStudio PBC | RRID:SCR_000432 | |
| Other | ProLong Diamond | Invitrogen | Cat. #: P36961 |