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. Author manuscript; available in PMC: 2022 Jan 1.
Published in final edited form as: Science. 2020 Nov 26;371(6524):eabd1673. doi: 10.1126/science.abd1673

Fig. 4. NusA interactions.

Fig. 4.

(A) Comparison of βFT-NusANTD interactions in the primed and engagement complexes, after superposition of NusANTDs. (B) ρ5/ρ6/NusAKH1 interaction network in the primed complex. (C) Correlation of accommodation of distal uDNA on the β’ZBD and NusA rotation underneath ρ6 in the primed complex. (D) NusA (“A”) effects on termination by WT RNAP, or RNAP variants lacking αCTDs or ω; dashed lines indicate spliced images. The RNA fractions are means ± SD of three independent experiments. ns, not significant; * P<0.1; ** P<0.001; *** P<0.0001.