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. Author manuscript; available in PMC: 2022 Feb 3.
Published in final edited form as: Neuron. 2020 Dec 1;109(3):488–501.e4. doi: 10.1016/j.neuron.2020.11.009

Figure 7. NMDARs containing insertions in GluN1 or GluN2A S2-M4 linkers only activate through the fast pathway.

Figure 7.

(A & B) Overlaid AMPAR structures of αK, S2-M4, and the top of M4, displayed as in Figures 3A & 3B except that the open state is green (D1-associated).

(C) Current traces from an outside-out patch of a single NMDAR containing an insertion in the GluN2A S2-M4 (V807+1G). See Figure 2A for details.

(D & E) Dwell time histograms for GluN1 S2-M4 (S802+1G) (D) or GluN2A S2-M4 (V807+1G) (E). Histograms best fit by single exponentials.

(F-H) Data displayed and analyzed as in Figures 3E–3G.

(F) Success rates for wild type, GluN1 S2-M4+1G (10 total patches) (global success rate, 0.99; 304 out of 304 trials), and GluN2A S2-M4+1G (7 total patches) (global success rate, 0.97; 213 out of 219 trials). ***p < 0.001, t-test.