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. 2021 Jan 21;5(2):197–212. doi: 10.7150/ntno.53888

Figure 7.

Figure 7

GSH depletion by BSO strongly hampers the intracellular accumulation of GSH-NSs. (A) Western blotting assessing the intracellular GSH content in OVCAR3 and MDAMB231 cells in the absence/presence of buthionine sulfoximine (BSO), an inhibitor of gamma-glutamylcysteine synthetase. For loading control, the membranes were re-probed with GAPDH. Graphs represent the densitometric analysis of three independent experiments. Data are presented as average (A.U., arbitrary units) ±SD. Significance was considered as follows: *p < 0.05; ***p < 0.001. (B-C) Cell were plated on sterile coverslips. Cell internalization studies were performed on OVCAR3 (B) and MDAMB231 cells (C) pre-treated or not with BSO and then incubated with C-6-GSH-NSs for 24 hours. Graphs representing the quantification of fluorescence intensity (INT.DEN. average per cell) ±SD are shown. Significance was considered as ****p < 0.0001.