Figure 8.

GSH depletion by BSO prevents RV-mediated toxic effects in both cancer cell models. Cells were plated on sterile coverslips, pre-treated or not with BSO and then incubated with RV-GSH-NSs for 24 and 48 hours. (A-B) Cells were labeled with Cell Tracker Blue fluorescent dye. Coverslips were washed and mounted on glasses and imaged immediately at the fluorescent microscope. Staining was performed in OVCAR3 (A) and MDAMB231 cells (B). Graphs represent the quantification of fluorescence intensity (INT.DEN. average per cell) ± SD. Representative images of three independent experiments are shown. Significance was considered as follows: *p < 0.05; **p < 0.01; ****p < 0.0001. (C-D) Cells were stained with propidium iodide (PI). Coverslips were washed and mounted on glasses and imaged immediately at the fluorescent microscope. Staining was performed in OVCAR3 (C) and MDAMB231 cells (D). Cell death was assessed by counting the percentage of PI positive cells and represented in the graphs (% ± SD). Representative images of three independent experiments are shown. Significance was considered as follows: ***p < 0.001; ****p<0.0001.