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A gene regulatory network for Ciona notochord fate specification. Bold lines indicate new cis-regulatory relationships in the Ciona notochord GRN identified or validated here. The dotted arrow from Mnx is supported by overexpression results but remains to be confirmed by loss of function. Other regulatory interactions in the Ciona notochord GRN have been described previously (Imai et al., 2002a,b, 2006; Yagi et al., 2004; Yasuo and Hudson, 2007).
