Fig. 1.
Preliminary verification of PLCR. (A) The cleavage efficiency of PfAgo guided by DNA guides of different lengths. (B) Normalized PLCR photoluminescent (PL) signal from SARS-CoV-2 RNA/HPV11 plasmid positive (1 pM/sample) and negative control (ddH2O) samples. (C) SARS-CoV-2 genome map showing PLCR target sequences. (D) Normalized two-channel PLCR photoluminescent (PL) signal from N and ORF1ab gene of SARS-CoV-2. Data are presented as mean ± SD of three technical replicates.