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. 2021 Jan 28;10:e60710. doi: 10.7554/eLife.60710

Video 13. CK-666 mediated Arp2/3 inhibition does not prevent cortical stress fiber assembly.

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Zoom in of a human osteosarcoma (U2OS) cell expressing LifeAct-TagGFP2 (gray) and miRFP670-paxillin (magenta) migrating on a fibronectin (10 µg/ml)-coated high-precision imaging dish after 4 hr treatment with 100 µM CK-666. Related to Figure 4—figure supplement 2C. Time-lapse ring-TIRFM video was recorded with Deltavision OMX SR with 30 s imaging interval. Playback rate 20 frames/s. Scale bar 5 µm.