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. Author manuscript; available in PMC: 2021 Jun 15.
Published in final edited form as: Oncogene. 2020 Dec 15;40(6):1106–1117. doi: 10.1038/s41388-020-01585-5

Figure 3. FXXLF motif is critical for AR-V7/-FL heterodimerization.

Figure 3.

LNCaP cells stably expressing cumate-inducible FLAG-tagged AR-V7WT or AR-V7MT were evaluated for interaction between AR-FL and AR-V7 using proximity ligation assays (PLA) under conditions of vehicle control or cumate stimulation in androgen-deprived condition. An AR C-terminal antibody and a FLAG-targeted antibody were used to track AR-FL and AR-V7, respectively. DAPI was used to stain the nucleus. These data indicate that AR-V7WT, AR-V7D, and AR-V7P, but not AR-V7F or AR-V7FD, can interact with AR-FL. The enlarged images in the inset show the interactions in a representative single cell. *, P < 0.001 from control.