Table 1.
The results of ANOVA for continuous variables, calculated for two EM clusters that divide 60 tissue samples, selected from the data of RNA expression (https://biogps.org/).[ 31 , 32 ]
| RNA expression | ANOVA for continuous variables | ||||||
|---|---|---|---|---|---|---|---|
| RAS component gene | Dataset name | Between sum of squares | df | Within sum of squares | df | F | P value |
| “Constriction” EM clusters from Figure 1 (18 tissue samples of high and 42 samples of low expression) | |||||||
| ProreninRec | 201442_s_at | 37.7520714 | 1 | 69.8697619 | 58 | 31.33859 | 0.00000 |
| ACE | 209749_s_at | 159.075627 | 1 | 202.094206 | 58 | 45.65389 | 0.00000 |
| AT1R | 208016_s_at | 1172.95753 | 1 | 1411.7723 | 58 | 48.18875 | 0.00000 |
| “Perfusion” EM clusters from Figure 2 (21 tissue samples of high and 39 samples of low expression) | |||||||
| Renin | 206367_at | 20 | 1 | 58 | 58 | 20.26553 | 0.00003 |
| ReninBP | 206617_s_at | 31 | 1 | 121 | 58 | 14.67651 | 0.00032 |
| ProreninRec | 201442_s_at | 24 | 1 | 84 | 58 | 16.39999 | 0.00015 |
| ACE | 209749_s_at | 50 | 1 | 311 | 58 | 9.27032 | 0.00350 |
| AT2R | 207293_s_at | 5 | 1 | 22 | 58 | 12.07863 | 0.00097 |
| ACE2 | 222257_s_at | 299 | 1 | 2413 | 58 | 7.18629 | 0.00955 |
| MAS1 | 208210_at | 40 | 1 | 296 | 58 | 7.75939 | 0.00721 |
| ET‐1 | 218995_s_at | 52415 | 1 | 385990 | 58 | 7.87601 | 0.00681 |
| ET‐1 RecA | 216235_s_at | 826 | 1 | 1700 | 58 | 28.19492 | 0.00000 |
| ET‐1 RecB | 204271_s_at | 32603 | 1 | 102602 | 58 | 18.43008 | 0.000068 |
| TSP1 | 201110_s_at | 1798934 | 1 | 20770724 | 58 | 5.02333 | 0.028850 |
“Constriction” EM clusters use only three genes, while “perfusion” clusters use ten genes. Only expressions of ACE and of prorenin receptors were found important in both instances of EM clustering.