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In vitro measurements of Intact and Total GIP levels show inconsistent results with varying assays and sample preparations. Measurements were performed with (A), Crystal Chem's Intact GIP ELISA kit, (C), Crystal Chem's Total GIP ELISAkit and (E), Millipores Total GIP ELISA kit. The different media contained either 0, 25 or 400 pmol/L mouse GIP(1-42). (B, D and F) show the recovery rates of these concentrations for the respective ELISA kits. Data are presented as mean ± SE, n = 4.
