Figure 1.

Human IgG and IgA bind HCMV gB and AD‐2. ELISA to determine IgG and IgA binding to gB and AD‐2 epitope of HCMV in (A) human serum (n = 24) and (B) human saliva (n = 24). All samples were diluted as indicated, and data are represented as OD450 nm values of triplicate determinations of three independent experiments. Horizontal dotted lines representing positive/negative cut‐off values for negative gB and negative AD‐2 samples were based on IgG gB‐negative samples (n = 4). Statistical differences comparing the mean OD450 nm values of IgG and IgA binding to gB and AD‐2 epitope at each dilution were obtained from the Mann–Whitney test (ns, not significant; ****P < 0.0001)