Figure 4.

Human milk‐derived IgG and IgA bind HCMV gB and AD‐2. (A) ELISA to determine IgG and IgA binding to gB and AD‐2 epitope of HCMV in human breastmilk samples (n = 14). Samples were diluted as indicated, and data are represented as OD450 nm values of triplicate determinations of three independent experiments. Horizontal dotted lines representing positive/negative cut‐off values for negative gB and negative AD‐2 samples were based on IgG gB‐negative samples (n = 4). Statistical differences comparing the mean OD450 nm values of IgG and IgA binding to gB and AD‐2 epitope at each dilution in part A were obtained from the Mann–Whitney test (ns, not significant; **P < 0.01; ***P < 0.001). (B) Scatterplot correlations of individual undiluted samples displaying IgG and IgA binding to both HCMV gB and the AD‐2 epitope. Horizontal and vertical dotted lines are shown representing positive/negative cut‐off values for negative gB and negative AD‐2 samples, respectively, and were based on IgG gB‐negative samples (n = 4). Correlation coefficients (Spearman's) and statistical significance for each set of determinations comparing IgG or IgA binding to gB and AD‐2 epitope are displayed within each graph