TABLE 1.
Rates of spontaneous and induced prion formation and propagationa
| Transgeneb | Rate of spontaneous prion formation at no. of days after transfection: |
Total no. monitored | Rate of induced prion formation |
||||||
|---|---|---|---|---|---|---|---|---|---|
| 5 |
11 |
19 |
|||||||
| Diffuse (n) | Foci (n [%]) | Diffuse (n) | Foci (n [%]) | Diffuse (n) | Foci (n [%]) | Diffuse (n) | Foci (n [%]) | ||
| HELLP(214-271)-RFP | 27 | 7 (21) | 13 | 21 (62) | 2 | 32 (94) | 34 | 0 | 24 (100) |
| HELLP(214-271)-GFP | 40 | 7 (15) | 24 | 23 (49) | 8 | 39 (83) | 47 | 0 | 24 (100) |
| GFP-HELLP(214-271) | 11 | 22 (67) | 1 | 32 (97) | 0 | 33 (100) | 33 | 0 | 24 (100) |
| RIP3(444-469)-RFP | 18 | 21 (54) | 8 | 31 (80) | 2 | 37 (95) | 39 | 0 | 24 (100) |
| RIP3(444-469)-GFP | 30 | 16 (35) | 15 | 31 (67) | 4 | 42 (91) | 46 | 0 | 24 (100) |
| GFP-RIP3(524-551) | 8 | 17 (68) | 3 | 22 (88) | 0 | 25 (100) | 25 | 0 | 24 (100) |
| RIP1(524-551)-RFP | 23 | 3 (12) | 14 | 12 (46) | 10 | 16 (62) | 26 | 0 | 24 (100) |
| RIP1(524-551)-GFP | 27 | 0 (0) | 25 | 2 (7) | 23 | 4 (15) | 27 | 0 | 24 (100) |
| GFP-RIP1(524-551) | 38 | 0 (0) | 36 | 2 (5) | 35 | 3 (8) | 38 | 0 | 24 (100) |
| PNT1(1-31)-GFP | 18 | 7 (28) | 1 | 24 (96) | 25 | 0 | 24 (100) | ||
| PNT1(1-31)-RFP | 17 | 8 (32) | 0 | 25 (100) | 25 | 0 | 24 (100) | ||
a
For each transgene, between 25 to 47 different transformants were monitored by fluorescence microscopy for foci formation over 19 days. After 60 days, 100% of the transformants (except RIP1-GFP [33%] and GFP-RIP1 [16%]) displayed dots. At least two other transformation experiments were achieved for each transgene and generated 100% of tested transformants (n > 10) with foci in fluorescence after 60 days, except for RIP1-GFP (20%) and GFP-RIP1 (15%).
b
RFP, red fluorescent protein; GFP, green fluorescent protein.