TABLE 2.

Growth alteration of strains coexpressing HELLP, CgHELLP, or HET-S cell death-inducing proteins and different prion-forming domains prior to and after prion infection

Coexpressed transgenes (PFD/cell death inducer)	No. of transformants analyzed	Growth before infection								Growth after infection
		Normal		Altered		Sublethal		Total abnormal		Normal		Altered		Sublethal		Total abnormal
		n	%	n	%	n	%	n	%	n	%	n	%	n	%	n	%
GFP-RIP3(444-469)/HELLP-RFP	15	14	93	1	7	0	0	1	7	3	20	9	60	3	20	12	80
RIP3(444-469)-RFP/HELLP-GFP	29	26	90	2	7	1	3	3	10	5	17	17	59	7	24	24	83
GFP-RIP3(444-469)/HET-S-RFP	18	16	89	1	6	1	6	2	11	15	83	2	11	1	6	3	17
RIP3(444-469)-RFP/HET-S-GFP	20	18	90	2	10	0	0	2	10	17	85	2	10	1	5	3	15
GFP-RIP1(524-551)/HELLP-RFP	36	31	86	3	8	2	6	5	14	7	19	20	56	9	25	29	81
RIP3(444-469)-RFP/HELLP-GFP	30	27	90	2	7	1	3	3	10	6	20	18	60	6	20	24	80
GFP-RIP1(524-551)/HET-S-RFP	18	16	89	1	6	1	6	2	11	15	83	3	17	0	0	3	17
RIP1(524-551)-RFP/HET-S-GFP	16	15	94	1	6	0	0	1	6	14	88	2	13	0	0	2	13
GFP(RIP3(444-459)/CgHELLP-RFP	39	35	90	4	10	0	0	4	10	6	15	22	56	11	28	33	85
GFP-RIP1(524-551)/HELLP-RFP	27	24	89	3	11	0	0	3	11	5	19	16	59	6	22	22	81
GFP-HELLP(214-271)/HELLP-GFP	18	15	83	2	11	1	6	3	17	3	17	10	56	5	28	15	83
HELLP(214-271)-RFP/HELLP-GFP	12	10	83	1	8	1	8	2	17	2	17	6	50	4	33	10	83
GFP-HELLF(209-277)/HELLP-RFP	34	31	91	1	3	1	3	2	6	28	82	3	9	3	9	6	18
HELLF(209-277)-RFP/HELLP-GFP	16	14	87	1	6	1	6	2	13	13	81	2	13	1	6	3	19
HET-s-RFP/HELLP-GFP	16	15	94	0	0	1	6	1	6	14	88	1	6	1	6	2	13